Journal: EMBO Reports

Article Title: Reporter‐based fate mapping in human kidney organoids confirms nephron lineage relationships and reveals synchronous nephron formation

doi: 10.15252/embr.201847483

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: Imaging was performed in glass‐bottomed dishes (MatTek) with glycerol submersion using either the Zeiss LSM 780 or Dragonfly Spinning Disk confocal microscope. table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Name Source Identifier Mouse monoclonal anti‐TRA‐1‐81 BioLegend Cat#330706 Mouse monoclonal anti‐SSEA‐4 BioLegend Cat#330408 Mouse monoclonal anti‐CD9 BD Biosciences Cat#555371 Rabbit monoclonal anti‐OCT4 Abcam Cat# {"type":"entrez-nucleotide","attrs":{"text":"Ab181557","term_id":"52839581","term_text":"AB181557"}} Ab181557 Rabbit polyclonal anti‐RFP (and mCherry) MBL Medical & Biological Laboratories Cat#PM005 Biotinylated Lotus Tetragonolobus Lectin Vector Laboratories Cat#B‐1325 Rabbit polyclonal anti‐SIX1 Cell Signaling Cat#12891 Rabbit polyclonal anti‐SIX2 Proteintech Group Cat#11562‐1‐AP Mouse monoclonal anti‐MEIS1/2/3 Active Motif Cat#39795 Mouse anti‐EPCAM (Alexa Fluor 488‐conjugated) BioLegend Cat#324210 Mouse anti‐EPCAM (Alexa Fluor 647‐conjugated) BioLegend Cat#324212 Mouse monoclonal anti‐GATA3 Thermo Fisher Scientific Cat#MA1‐028 Goat polyclonal anti‐GATA3 R&D Systems Cat#AF2605 Sheep polyclonal anti‐NEPHRIN R&D Systems Cat#AF4269 Mouse monoclonal anti‐CD31 BD Pharmingen Cat#550274 Mouse monoclonal anti‐E‐CADHERIN BD Biosciences Cat#610181 Chicken polyclonal anti‐GFP Abcam Cat#Ab13970 Open in a separate window Antibodies used in this study

Techniques: Plasmid Preparation

Journal: Journal of the American Society of Nephrology

Article Title: Podocyte-Specific Overexpression of the Antioxidant Metallothionein Reduces Diabetic Nephropathy

doi: 10.1681/asn.2007080967

Figure Lengend Snippet: Figure 4. Altered expression of slit diaphragm proteins in isolated glomeruli. (A) Nephrin, podocalyxin, and GAPDH protein in glomeruli; FVB (F), OVE26 (O), and OVE26Nmt7 (ON). (B and C) Expression of nephrin (B) and podocalyxin (C), normalized to GAPDH expression. OVE26 and OVE26Nmt7 are always less than FVB (*) and podocalyxin OVE26Nmt7 is greater than OVE26 (**) (P 0.05 by Kruskal Wallis ANOVA).

Article Snippet: Antibodies used were nephrin (1:1000, guinea pig; Fitzgerald, Concord, MA), podocalyxin (1:3000; Alpha Diagnos- tic Int.

Techniques: Expressing, Isolation

Journal: Journal of Translational Autoimmunity

Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

doi: 10.1016/j.jtauto.2025.100307

Figure Lengend Snippet: Schematic overview of the workflow for the characterization of anti-nephrin autoantibodies . Panel A. Paired serum and urine samples from 20 healthy donors, 100 NS patients were analyzed using an ELISA assay to detect anti-nephrin autoantibodies. Panel B. The same paired samples were analyzed using a pull-down assay to verify whether the identified anti-nephrin positive samples could also immunoprecipitate the extracellular domain of human nephrin. Panel C. The fucose content of IgG from the same serum samples was measured using an ELISA assay using the fucose-binding lectins. INS, Idiopathic nephrotic syndrome; FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis; SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS.

Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Pull Down Assay, Binding Assay

Journal: Journal of Translational Autoimmunity

Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

doi: 10.1016/j.jtauto.2025.100307

Figure Lengend Snippet: Circulating antibodies against the extracellular domain of nephrin in idiopathic nephrotic syndrome (INS) patients . Panel A shows the levels of circulating anti-nephrin antibodies measured by ELISA in 10 healthy donors and 100 INS patients stratified based on biopsy. FSGS, focal segmental glomerulosclerosis; MCD, minimal change disease; MN, membranous nephropathy; LN, lupus nephritis. Additionally, in patients with FSGS and MCD, anti-nephrin levels were further stratified based on the type of INS. SDNS, steroid-dependent NS; MDNS, multidrug-dependent NS; MRNS, multidrug-resistant NS. Panel B shows the titer of immunoprecipitating anti-nephrin antibodies in the serum and urine of the same subjects as in panel A. Panel C shows the prevalence of circulating anti-nephrin autoantibodies in the investigated cohorts of children and young adults with INS, with red and light gray colors representing positive and negative percentages, respectively. Panel D shows the prevalence of circulating anti-nephrin autoantibodies in FSGS and MCD samples stratified based on their pharmacological response. The dotted line indicates the statistically significant threshold.

Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Translational Autoimmunity

Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

doi: 10.1016/j.jtauto.2025.100307

Figure Lengend Snippet: Correlation between anti-nephrin antibody titers and urinary proteinuria in patients with anti-nephrin-associated MCD or FSGS . Panels A and B show the correlation between anti-nephrin antibody titers in serum, measured by ELISA (A) or immunoprecipitation (B), and 24-h proteinuria. Panels C and D show the corresponding correlation between urinary anti-nephrin antibody titers, measured by ELISA (C) or immunoprecipitation (D), and proteinuria. R = Spearman's correlation coefficient.

Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Immunoprecipitation

Journal: Journal of Translational Autoimmunity

Article Title: Afucosylated IgG in idiopathic nephrotic syndrome patients with anti-nephrin autoantibodies correlate with disease activity

doi: 10.1016/j.jtauto.2025.100307

Figure Lengend Snippet: Evaluation of fucose in IgG by enzyme-linked immunosorbent assay (ELISA) with biotinylated lectins. Panels A and B show the levels of core-fucosylated IgG (α1,6-linked fucose) and antennary-fucosylated IgG (α1,2-linked fucose), respectively, measured using AAL and UEA-I lectins in the serum of healthy donors and NS patients, stratified by circulating anti-nephrin autoantibodies. Panels C and D display the inverse correlation between 24-h proteinuria and serum IgG fucosylation levels, as measured by AAL (Panel C) and UEA-I (Panel D), in patients with anti-nephrin–associated MCD or FSGS. Panels E and F show the corresponding levels of core and antennary fucosylation in IgG from urine samples, measured by AAL and UEA-I, and stratified by anti-nephrin antibody status. Red dots indicate patients positive for anti-nephrin autoantibodies, as confirmed by both ELISA and immunoprecipitation.

Article Snippet: Positive and negative controls included 20 ng of sheep anti-human nephrin IgG (R&D Systems Cat# AF4269) and rabbit anti-human albumin IgG (Merck Cat# A3293), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Immunoprecipitation

Journal: Medicine

Article Title: Association of microRNA-155, interleukin 17A, and proteinuria in preeclampsia

doi: 10.1097/MD.0000000000006509

Figure Lengend Snippet: (A–C) Urine nephrin expression at different urine protein levels.

Article Snippet: Sections were incubated with a primary polyclonal goat anti-human nephrin antibody against the intracellular domain of nephrin (1:50; R&D Systems), then with a secondary gold-conjugated (10 nm) rabbit anti-goat secondary antibody (1:100; DAKO, DK).

Techniques: Expressing

Journal: Journal of the American Society of Nephrology : JASN

Article Title: The Calcium-Dependent Protease Calpain-1 Links TRPC6 Activity to Podocyte Injury

doi: 10.1681/ASN.2016111248

Figure Lengend Snippet: Reduced calpastatin, increased Transient Receptor Potential channel C6 (TRPC6) expression, and enhanced calcineurin activation in patients with FSGS. (A) Representative pictures of costaining of the endogenous calpain inhibitor calpastatin and nephrin in healthy controls and patients with FSGS. (B) Calpastatin expression in podocytes was determined by quantifying the costaining of calpastatin and nephrin. (C) Cortical calcineurin activity, as a downstream calpain target, was determined as well as (D) cortical TRPC6 mRNA and (E) glomerular TRPC6 protein expression as quantified by immunofluorescence costained with synaptopodin. FSGS, n=3; control, n=5. *P<0.05 compared with healthy controls.

Article Snippet: Immunofluorescence Analyses Immunofluorescence staining for TRPC6 (Abcam, Cambridge, United Kingdom and Alomone, Jerusalem, Israel), Talin-1 (Acris Antibodies, Herford, Germany), nephrin (R&D, Minneapolis, MN), synaptopodin (Santa Cruz), calpastatin (Santa Cruz), and desmin (Santa Cruz) was performed on 2- μ m cryosections of rat and human kidney cortex.

Techniques: Expressing, Activation Assay, Activity Assay, Immunofluorescence, Control